Isolation, purification, and immunomodulatory activity in vitro of three polysaccharides from roots of Cudrania tricuspidata.
نویسندگان
چکیده
Three water-soluble polysaccharides (CTPS-1A, CTPS-2B, and CTPS-3A) were obtained from roots of Cudrania tricuspidata (Carr.) Bur. in this study. The homogeneity of polysaccharides was determined, and the average molecular weight, ultraviolet, infrared, monosaccharide composition, and methylation analyses were carried out. Immunomodulatory activity assays in vitro showed that the three polysaccharides could directly stimulate the proliferation of mouse splenocytes alone or combining with concanavalin A or lipopolysaccharide. Furthermore, their stimulating activities were higher than that of the widely clinically used lentinan at optimal concentrations. CTPS-1A and CTPS-2B also enhanced the pinocytic activity of mouse peritoneal macrophages.
منابع مشابه
Protein Tyrosine Phosphatase 1B Inhibitors from the Roots of Cudrania tricuspidata.
A chemical investigation of the methanol extract from the roots of Cudrania tricuspidata resulted in the isolation of 16 compounds, including prenylated xanthones 1-9 and flavonoids 10-16. Their structures were identified by NMR spectroscopy and mass spectrometry and comparisons with published data. Compounds 1-9 and 13-16 significantly inhibited PTP1B activity in a dose dependent manner, with ...
متن کاملInhibitory Activities of Cudrania tricuspidata Leaves on Pancreatic Lipase In Vitro and Lipolysis In Vivo
To identify effective herb to treat obesity, we screened 115 herbal extracts for inhibition of porcine pancreatic lipase (triacylg-ycerol acylhydrolase, EC 3.1.1.3) activity in vitro. Of the extracts tested, Cudrania tricuspidata leaves exhibited the most pronounced inhibitory effect on lipase activity with an IC(50) value of 9.91 μg/mL. Antilipid absorption effects of C. tricuspidata leaves we...
متن کاملQuantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts.
Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-per...
متن کاملComparison of pancreatic lipase inhibitory isoflavonoids from unripe and ripe fruits of Cudrania tricuspidata
The composition and content of the active constituents and their biological activity vary according to diverse factors including their maturation stages. A previous study showed that the fruits of Cudrania tricuspidata inhibited pancreatic lipase activity, a key enzyme in fat absorption. In this study, we investigated the chemical composition and pancreatic lipase inhibitory activity of unripe ...
متن کاملHistone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
Objective(s): Histone deacetylase inhibitory and cytotoxic activities of 18 naturally occuring terpenoids (ferutinin, stylosin, tschimgine and guaiol), coumarins (umbelliprenin, farnesiferone B, conferone, feselol, ligupersin A, conferdione, conferoside) and sulfur-containing derivatives (latisulfies A-E, persicasulphides A and C) from the roots of three species of Ferula (Ferula latisecta, Fer...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Acta biochimica et biophysica Sinica
دوره 43 5 شماره
صفحات -
تاریخ انتشار 2011